Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Boney KO[original query] |
---|
Population diversity among Bordetella pertussis isolates, United States, 1935-2009
Schmidtke AJ , Boney KO , Martin SW , Skoff TH , Tondella ML , Tatti KM . Emerg Infect Dis 2012 18 (8) 1248-55 Since the 1980s, pertussis notifications in the United States have been increasing. To determine the types of Bordetella pertussis responsible for these increases, we divided 661 B. pertussis isolates collected in the United States during 1935-2009 into 8 periods related to the introduction of novel vaccines or changes in vaccination schedule. B. pertussis diversity was highest from 1970-1990 (94%) but declined to approximately 70% after 1991 and has remained constant. During 2006-2009, 81.6% of the strains encoded multilocus sequence type prn2-ptxP3-ptxS1A-fim3B, and 64% were multilocus variable number tandem repeat analysis type 27. US trends were consistent with those seen internationally; emergence and predominance of the fim3B allele was the only molecular characteristic associated with the increase in pertussis notifications. Changes in the vaccine composition and schedule were not the direct selection pressures that resulted in the allele changes present in the current B. pertussis population. |
Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens.
Tatti KM , Sparks KN , Boney KO , Tondella ML . J Clin Microbiol 2011 49 (12) 4059-66 A novel multi-target real-time PCR (R-PCR) assay for the rapid identification and speciation of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using multi-copy insertion sequences (IS) in combination with the pertussis toxin subunit S1 (ptxS1) singleplex assay. The R-PCR targets for the multiplex assay include IS481 commonly found in B. pertussis and B. holmesii, IS1001 of B. parapertussis, and the IS1001-like sequence of B. holmesii. Overall, 402 Bordetella spp. and 66 non-Bordetella spp. isolates were tested in the multi-target assay. Cross-reactivity was found only with 5 B. bronchiseptica isolates which were positive with IS1001 of B. parapertussis. The lower limit of detection (LLOD) of the multiplex assay was similar to the LLOD of each target in an individual assay format which was approximately 1 genomic equivalent per reaction for all targets. A total of 197 human clinical specimens obtained during cough-illness outbreak investigations were used to evaluate the multi-target R-PCR assay. The multiplex assay results from 87 clinical specimens were compared to the individual R-PCR assays and culture. The multi-target assay is useful as a diagnostic tool to confirm B. pertussis infections and to rapidly identify other Bordetella species. In conclusion, the use of this multi-target R-PCR approach increases specificity, while decreasing the amount of time, reagents, and specimen necessary for R-PCR reactions used for accurate diagnosis of pertussis-like illness. |
- Page last reviewed:Feb 1, 2024
- Page last updated:May 06, 2024
- Content source:
- Powered by CDC PHGKB Infrastructure